Translational_Unit
OsPHT1;6 T

Part:BBa_K4213043:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-04)


TetO7:pTriple:OsPHT1;6(partial-opt):Venus:tNOS(stuffer)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal BamHI site found at 2132
    Illegal BamHI site found at 2886
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 2804
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GoldenBraid 2.0


Source

-

References